General Description Department of Medicine, Division of Infectious Diseases
Work Schedule/Hours: Monday-Friday/8:30am-5:00pm
Under the general supervision of a faculty investigator or laboratory supervisor, the Research Technician will focus on performing both routine immunological assays and experimental techniques in the study of hepatitis B virus (HBV). He/she will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
Duties and Responsibilities:
Follow established procedures and utilizes aseptic technique to perform experiments associated with HBV and HIV immunology and molecular biology. Includes but not limited to:
Perform routine immunology and molecular biology assays to explore the immune response to an acute hepatitis B infection including ELISAs, ELISpot, flow cytometry, intracellular staining, PCR, immunohistochemistry, gel electrophoresis, transfections, and western blots. Assist with maintenance of cell and serum repository for the study, which requires blood processing.
Operate basic laboratory equipment such as centrifuge, pH meter, analytical balance, scintillation counter, light microscope, plate washer and/or utilize biological safety cabinet.
This person may also utilize sophisticated instrumentation such as flow cytometer, spectrophotometer, luminometer, thermal cycler and laboratory automation solutions/robotics. Responsible for operation of lab computers for data entry.
Maintain accurate data records and laboratory notebook. Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Along with the laboratory supervisor, works to develop and institute protocols for routine laboratory analyses. Maintains and improves training procedures and is responsible for training entry-level employees on relevant techniques.
This description is a general statement of required duties and responsibilities performed on a regular and continuous basis. It does not exclude other duties as assigned.