Department of Medicine, Division of Infectious Diseases Work Schedule/Hours: Monday-Friday/8:30am-5:00pm
Under the general supervision of a faculty investigator or laboratory supervisor, the Research Technician will focus on performing both routine immunological assays and experimental techniques in the study of hepatitis B virus (HBV). He/she will be expected to organize and present data to the faculty investigator. Additional responsibilities will include contributing to general maintenance of the laboratory, equipment, and supplies.
Duties and Responsibilities:
Follow established procedures and utilizes aseptic technique to perform experiments associated with HBV and HIV immunology and molecular biology. Includes but not limited to:
Perform routine immunology and molecular biology assays to explore the immune response to an acute hepatitis B infection including ELISAs, ELISpot, flow cytometry, intracellular staining, PCR, immunohistochemistry, gel electrophoresis, transfections, and western blots. Assist with maintenance of cell and serum repository for the study, which requires blood processing.
This person may also utilize sophisticated instrumentation such as flow cytometer, spectrophotometer, luminometer, thermal cycler and laboratory automation solutions/robotics. Responsible for operation of lab computers for data entry.
Maintain accurate data records and laboratory notebook. Maintains and makes necessary adjustments to equipment, e.g. calibrating, alignment. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Along with the laboratory supervisor, works to develop and institute protocols for routine laboratory analyses. Maintains and improves training procedures and is responsible for training entry-level employees on relevant techniques.
This description is a general statement of required duties and responsibilities performed on a regular and continuous basis. It does not exclude other duties as assigned.
Bachelor's degree in biology, chemistry, or related field required. Some related post-undergraduate work experience preferred.
Special Knowledge, Skills, and Abilities: Basic techniques in virology, immunology, and molecular biology.
Some related post-undergraduate work experience. Minimum 1 year of experience. Familiar with basic tissue culture techniques such as preparing stock solutions, buffers and media and passing cell lines. Experience with mouse handling and procedures a plus.
Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130... years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Baltimore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.