Berkeley Lab's Environmental Genomics and Systems BiologyDivision has an opening for a Scientific Engineering Associate. Under general supervision, you will perform and supervise non-exempt staff in the development and application of mass spectrometry-related high throughput workflows for analyzing microbial metabolites and secondary metabolite production. You will be advancing and applying high throughput applications integrating advanced technologies including robotic automation, microfluidics, acoustic printing, with rapid mass spectrometry analysis. You will have additional responsibilities for preparation of engineered microbial strains, protein expression, and purification for high throughput characterization using mass spectrometry based approaches. Critical to the success of this position, you will be working closely with internal and external collaborators, to customize experimental plans for mass spectrometry analysis of specific research samples, analyze and integrated the results, assist in writing proposals, and preparing manuscripts and reports. This position reports to the Metabolomics group lead.
Apply engineering, scientific and/or other technical concepts to develop LC-MS, MALDI and other mass spectrometry-related workflows for analyzing microbial metabolites and secondary metabolite production.
Develop, optimize and standardize mass spectrometry instrumentation methods for targeted and untargeted analysis of microbial metabolism, with exometabolomics being a primary focus.
Develop, optimize and standardize sample preparation and extraction methods specific for targeted classes of molecules of interest (e.g. secondary metabolites, lipids, amino acids, etc) and microbial species, and transfer methods for high-throughput mass spectrometry by integrating liquid handling robotic automation
Innovate novel extraction protocols and mass spec methods for problematic, non-standard samples (e.g. environmental samples such as soils w/ high salt, low concentration, acidic pH)
Implement new technology and new protocols into production
Operate, maintain and troubleshoot mass spectrometry instrumentation
Sample preparation, extraction and analysis
Work closely with internal and external collaborators, customize experimental plans for mass spectrometry analysis of specific research samples. Process samples, analyze data and prepare report results for collaborators and publication.
Perform bioinformatics to integrate mass spectrometry-based metabolomics analysis with metagenomics and metatranscriptomics experiments and for functional genomics.
Contribute to scientific literature with publication of peer-reviewed journal articles.
Continuously develop metabolite atlases for rapid annotation and quantification of samples used in data analysis.
Effectively communicate with the other members in the Metabolomics group and provide implementation plans that includes timeline, procedures, expected outcomes, and risks.
Participate in cross-team and cross-group collaborative projects within the DOE JGI as well as users' projects.
Continuously provide input and suggestions in processing protocols, reagents and new automation protocols.
Summarize experimental results and present them at the group meeting.
Bachelor's degree in Analytical Chemistry, Biochemistry, Bioengineering or related discipline or equivalent relevant experience.
Significant technical expertise in mass spectrometry and a proven track record for:
Operating and maintaining mass spectrometry instrumentation
Developing and optimizing novel LC-MS methods.
Developing and optimizing novel extraction and sample preparation methods for a range of samples (microbes, soils, etc) and target metabolites (e.g. amino acids, carbohydrates, etc) for mass spectrometry analysis
Performing mass spectrometry data analysis using python
Deep experience applying metabolomics methods to studying soils
Deep expertise in high throughput laser-desorption ionization mass spectrometry
Experience using acoustic printing instrumentation
Experience with synthetic biology
Detailed and specific experience expressing proteins using IVTT or recombinant techniques and purifying proteins for mass spectrometry based enzymatic activities
Previous peer-reviewed publications in the field of mass spectrometry and/ or soil metabolomics, with strong demonstrated ability in writing, analysis and preparation of figures for presentations and publication
Experience in mass spectrometry, cell culture, analytical chemistry, wet lab work, development of novel protocols
Demonstrated experience applying technical, scientific and engineering principles to evaluate and design new manual mass spectrometry protocols and develop new workflows
Additional Desired Qualifications:
Advanced degree (MS/PhD) in Analytical Chemistry, Biochemistry, Bioengineering or related discipline.
Strong analytical skills to effectively evaluate data and present results.
Keep precise and detailed records and laboratory notebooks.
Excellent problem-solving and decision-making skills with the ability to provide resolutions to technical problems of complex scope where analysis of situations or data requires a review of identifiable factors.
Ability to successfully work independently and in a dynamic team environment (e.g. integrity, trust, teamwork, interpersonal skills, values differences, dealing with change and ambiguity, strategy, and written and oral communication).
Highly competent in the use of computers and related software programs; i.e., PC, Mac, and UNIX operating systems.
Excellent and effective interpersonal, communication and presentation skills with the ability to summarize experimental results and present at group meetings.
Ability to interact collaboratively with staff at all levels of the organization.
The posting shall remain open until the position is filled.
This is a full time 1-year term appointment with the possibility of extension or conversion to Career appointment based upon satisfactory job performance, continuing availability of funds and ongoing operational needs.
Full-time, M-F, exempt (monthly paid) from overtime pay.
Salary is commensurate with experience.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
DOE Joint Genome Institute (JGI) — 2800 Mitchell Dr., Walnut Creek, CA.
Learn About Us:
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 84588
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.